Hotspot

rpl16-rps3

LSC · 81169-82361 · 1193 bp

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Pi 0.00182
MarkerSeek score 55.50
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1193
variable_sites
21
pi
0.00182
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 CAAGTGCCTGAAGAGCGTAT GAGGGTAGGGTTCCACTACA 50.0 55.0 58.0 57.8 1420-1429 bp 1.0 79.7
rpl16-rps3_p2 CAAGTGCCTGAAGAGCGTAT ACTACAAACCATTCGCGCTA 50.0 45.0 58.0 57.6 1406-1415 bp 1.0 78.6
rpl16-rps3_p3 CAAGTGCCTGAAGAGCGTAT AGAGGGTAGGGTTCCACTAC 50.0 55.0 58.0 57.5 1421-1430 bp 1.0 77.2
rpl16-rps3_p4 CAAGTGCCTGAAGAGCGTAT TCCACTACAAACCATTCGCG 50.0 50.0 58.0 58.6 1409-1418 bp 1.0 77.1
rpl16-rps3_p5 GGGTTCAAGTGCCTGAAGAG GAGGGTAGGGTTCCACTACA 55.0 55.0 58.5 57.8 1425-1434 bp 1.0 76.0