Hotspot

petA-psbJ

LSC · 62634-63454 · 821 bp

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Pi 0.00654
MarkerSeek score 58.40
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
821
variable_sites
26
pi
0.00654
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GCGTACAAGGCCTTTTGTTC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.0 57.6 932-958 bp 1.0 81.4
petA-psbJ_p2 TGTTCTTCTTCGCAGCTGTT ATTCCGGATTGGGTTCATCC 45.0 50.0 58.0 57.6 917-943 bp 1.0 81.4
petA-psbJ_p3 TTGTTCTTCTTCGCAGCTGT ATTCCGGATTGGGTTCATCC 45.0 50.0 58.0 57.6 918-944 bp 1.0 81.4
petA-psbJ_p4 GGCCTTTTGTTCTTCTTCGC ATTCCGGATTGGGTTCATCC 50.0 50.0 57.9 57.6 924-950 bp 1.0 81.3
petA-psbJ_p5 ATTACGCGTACAAGGCCTTT ATTCCGGATTGGGTTCATCC 45.0 50.0 57.9 57.6 937-963 bp 1.0 80.9