Hotspot

psaA-ycf3

LSC · 41578-42319 · 742 bp

Back to Aldama

Pi 0.00298
MarkerSeek score 56.10
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
742
variable_sites
12
pi
0.00298
unique_haplotype_count
24
primer_available
yes
alignment_reliability
0.99
markerseek_score
56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GAGAAATGACCCGGTCTAGC AGCCGCTGAGTATTGGAAAC 55.0 50.0 57.8 58.3 600-953 bp 1.0 77.9
psaA-ycf3_p2 GAGAAATGACCCGGTCTAGC CAAGCCGCTGAGTATTGGAA 55.0 50.0 57.8 58.3 598-955 bp 1.0 77.9
psaA-ycf3_p3 GAGAAATGACCCGGTCTAGC AAGCCGCTGAGTATTGGAAA 55.0 45.0 57.8 57.5 597-954 bp 1.0 76.1
psaA-ycf3_p4 TTTTACTTCTGGTTCCGGCG AGCCGCTGAGTATTGGAAAC 50.0 50.0 58.5 58.3 535-888 bp 1.0 76.0
psaA-ycf3_p5 TTTTACTTCTGGTTCCGGCG CAAGCCGCTGAGTATTGGAA 50.0 50.0 58.5 58.3 533-890 bp 1.0 76.0