Hotspot

petN-psbM

LSC · 10424-10941 · 518 bp

Back to Aldama

Pi 0.00433
MarkerSeek score 48.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
518
variable_sites
10
pi
0.00433
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTT 50.0 40.0 58.2 55.6 680-701 bp 1.0 60.6
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT TGCTACTGCACTGTTCATTTT 50.0 38.1 58.2 56.4 680-701 bp 1.0 58.9
petN-psbM_p3 ATAGTAAGTCTCGCTTGGGC TGCTACTGCACTGTTCATTT 50.0 40.0 57.1 55.6 681-702 bp 1.0 54.7
petN-psbM_p4 CTCGCTTGGGCTGCTTTAAT TGCTACTGCACTGTTCATTT 50.0 40.0 58.9 55.6 672-693 bp 1.0 54.6
petN-psbM_p5 TGGGCTGCTTTAATGGTAGTC TGCTACTGCACTGTTCATTT 47.6 40.0 58.0 55.6 666-687 bp 1.0 53.8