Hotspot

trnF-GAA-ndhJ

LSC · 47379-47924 · 546 bp

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Pi 0.0395

MarkerSeek score 80.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 546
variable_sites: 67
pi: 0.0395
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	675-742 bp	1.0	90.4
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	711-778 bp	1.0	90.3
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	TGAAAGTTGGATAGGCTGGC	55.0	50.0	58.0	57.9	683-750 bp	1.0	90.3
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	647-714 bp	1.0	90.2
trnF-GAA-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	ATCCACGCCTGAAACGTATC	55.0	50.0	57.9	58.1	699-766 bp	1.0	89.4