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psbZ-trnG-GCC

LSC · 35348-35670 · 323 bp

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Pi 0.0347

MarkerSeek score 72.80

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 323
variable_sites: 32
pi: 0.0347
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGAAGGTTGGTCG	AATCGAACCCGCATCTTCTC	55.0	50.0	57.9	58.1	471-514 bp	1.0	88.0
psbZ-trnG-GCC_p2	CTTCTCCTGAAGGTTGGTCG	GAATCGAACCCGCATCTTCT	55.0	50.0	57.9	58.1	472-515 bp	1.0	88.0
psbZ-trnG-GCC_p3	TTCTCCTGAAGGTTGGTCGA	AATCGAACCCGCATCTTCTC	50.0	50.0	58.3	58.1	470-513 bp	1.0	86.4
psbZ-trnG-GCC_p4	TTCTCCTGAAGGTTGGTCGA	GAATCGAACCCGCATCTTCT	50.0	50.0	58.3	58.1	471-514 bp	1.0	86.4
psbZ-trnG-GCC_p5	TCCTGAAGGTTGGTCGAGTA	AATCGAACCCGCATCTTCTC	50.0	50.0	57.7	58.1	467-510 bp	1.0	86.4