Hotspot

trnT-GGU-psbD

LSC · 30735-32012 · 1278 bp

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Pi 0.0313

MarkerSeek score 80.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1278
variable_sites: 121
pi: 0.0313
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GCCCTTTTAACTCAGCGGTA	GGCCAGACCAACCTACAAAA	50.0	50.0	57.9	58.0	1454-1546 bp	1.0	86.9
trnT-GGU-psbD_p2	GCCCTTTTAACTCAGCGGTA	TAGGCCAGACCAACCTACAA	50.0	50.0	57.9	58.0	1456-1548 bp	1.0	86.7
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	GGCCAGACCAACCTACAAAA	50.0	50.0	58.3	58.0	1417-1509 bp	1.0	85.7
trnT-GGU-psbD_p4	GGCGTAAGTCATCGGTTCAA	TAGGCCAGACCAACCTACAA	50.0	50.0	58.3	58.0	1419-1511 bp	1.0	85.5
trnT-GGU-psbD_p5	GCCCTTTTAACTCAGCGGTA	ATAGGCCAGACCAACCTACA	50.0	50.0	57.9	57.7	1457-1549 bp	1.0	84.8