Hotspot

rps2-rpoC2

LSC · 15048-15257 · 210 bp

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Pi 0.0551

MarkerSeek score 75.30

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 210
variable_sites: 32
pi: 0.0551
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	TGCAGAGATATAGGGTGCCA	AAACTGCTCGGGTTTTAGCA	50.0	45.0	57.9	58.0	600-610 bp	1.0	87.6
rps2-rpoC2_p2	TTCCCGTCTTTTGTTCCGTT	AAACTGCTCGGGTTTTAGCA	45.0	45.0	58.0	58.0	874-884 bp	1.0	86.1
rps2-rpoC2_p3	ATCGTTGGCTGGAATCGAAA	AAACTGCTCGGGTTTTAGCA	45.0	45.0	57.9	58.0	1113-1123 bp	1.0	84.9
rps2-rpoC2_p4	TCGTTGGCTGGAATCGAAAT	AAACTGCTCGGGTTTTAGCA	45.0	45.0	57.9	58.0	1112-1122 bp	1.0	84.9
rps2-rpoC2_p5	TTCCCGTCTTTTGTTCCGTT	CGCAAGGTCTTCCGAAAGTA	45.0	50.0	58.0	57.9	1321-1331 bp	0.916667	80.2