Hotspot

trnR-UCU-atpA

LSC · 9334-9995 · 662 bp

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Pi 0.0549

MarkerSeek score 79.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 662
variable_sites: 95
pi: 0.0549
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	TCAGGACCAAATGGAACGTT	47.6	45.0	57.3	57.4	636-783 bp	1.0	73.3
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	TTCAGGACCAAATGGAACGT	47.6	45.0	57.3	57.4	637-784 bp	1.0	73.3
trnR-UCU-atpA_p3	TGGATAGGACAGAGGTCTTCT	TCAGGACCAAATGGAACGTT	47.6	45.0	56.9	57.4	671-818 bp	1.0	70.4
trnR-UCU-atpA_p4	TGGATAGGACAGAGGTCTTCT	TTCAGGACCAAATGGAACGT	47.6	45.0	56.9	57.4	672-819 bp	1.0	70.4
trnR-UCU-atpA_p5	GGAGTAAAAAGCGTCCATTGTC	TCAGGACCAAATGGAACGTT	45.5	45.0	57.9	57.4	696-843 bp	0.916667	66.8