Hotspot

rps16

LSC · 46643-47722 · 1080 bp

Back to Alcantarea

Pi 0.00278

MarkerSeek score 60.30

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1080
variable_sites: 3
pi: 0.00278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	AGCAAGAAAAAGGAAAATGGTGT	ACCGGTTGAACCAATGACTA	34.8	45.0	57.6	56.8	1271 bp	1.0	43.4
rps16_p2	AGCAAGAAAAAGGAAAATGGTGT	TACCGGTTGAACCAATGACT	34.8	45.0	57.6	56.8	1272 bp	1.0	43.4
rps16_p3	GCAAGAAAAAGGAAAATGGTGTG	ACCGGTTGAACCAATGACTA	39.1	45.0	57.6	56.8	1270 bp	1.0	43.0
rps16_p4	GCAAGAAAAAGGAAAATGGTGTG	TACCGGTTGAACCAATGACT	39.1	45.0	57.6	56.8	1271 bp	1.0	43.0
rps16_p5	GCAAGAAAAAGGAAAATGGTGT	ACCGGTTGAACCAATGACTA	36.4	45.0	56.2	56.8	1270 bp	1.0	40.2