Hotspot

rpoC1

LSC · 62314-65077 · 2764 bp

Back to Alcantarea

Pi 0.00145

MarkerSeek score 56.90

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2764
variable_sites: 4
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TTGGAAAAACCCGAATTCCG	TCGAGAACTACGATCTTTGGC	45.0	47.6	56.9	57.9	2921 bp	1.0	62.2
rpoC1_p2	TTGGAAAAACCCGAATTCCG	CGAGAACTACGATCTTTGGCT	45.0	47.6	56.9	57.8	2920 bp	1.0	62.2
rpoC1_p3	GGCATCGCCTAATCGAATTTC	TCGAGAACTACGATCTTTGGC	47.6	47.6	57.8	57.9	2901 bp	1.0	61.4
rpoC1_p4	GGCATCGCCTAATCGAATTTC	CGAGAACTACGATCTTTGGCT	47.6	47.6	57.8	57.8	2900 bp	1.0	61.4
rpoC1_p5	GGCATCGCCTAATCGAATTT	TCGAGAACTACGATCTTTGGC	45.0	47.6	56.6	57.9	2901 bp	1.0	60.1