Hotspot

petG-trnW-CCA

LSC · 109783-109904 · 122 bp

Back to Alcantarea

Pi 0.0328

MarkerSeek score 71.40

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 4
pi: 0.0328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	226 bp	1.0	80.0
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	CAAAACCCGATGTCGTAGGT	50.0	50.0	57.8	57.9	190 bp	1.0	79.7
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	CCCGATGTCGTAGGTTCAAA	50.0	50.0	57.8	57.6	185 bp	1.0	77.5
petG-trnW-CCA_p4	ACAGACGTGGTGATCAGTTG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	57.8	57.7	224 bp	1.0	77.4
petG-trnW-CCA_p5	ACAGACGTGGTGATCAGTTG	TCCAAAACCCGATGTCGTAG	50.0	50.0	57.8	57.6	192 bp	1.0	77.4