Hotspot

petL-petG

LSC · 109492-109668 · 177 bp

Back to Alcantarea

Pi 0

MarkerSeek score 29.80

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 177
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	CGGTTTTCTACTGGCTGCTT	CAACTGATCACCACGTCTGT	50.0	50.0	58.5	57.8	355 bp	1.0	75.0
petL-petG_p2	TTCGGTTTTCTACTGGCTGC	CAACTGATCACCACGTCTGT	50.0	50.0	58.5	57.8	357 bp	1.0	74.9
petL-petG_p3	CGGTTTTCTACTGGCTGCTT	CCAACTGATCACCACGTCTG	50.0	55.0	58.5	58.6	356 bp	1.0	71.7
petL-petG_p4	CGGTTTTCTACTGGCTGCTT	AAGGTCCAACTGATCACCAC	50.0	50.0	58.5	57.4	361 bp	1.0	71.6
petL-petG_p5	CGGTTTTCTACTGGCTGCTT	GTCCAACTGATCACCACGTC	50.0	55.0	58.5	58.6	358 bp	1.0	71.6