Hotspot

trnF-GAA-ndhJ

LSC · 90645-91423 · 779 bp

Back to Alcantarea

Pi 0.00257

MarkerSeek score 52.70

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 779
variable_sites: 2
pi: 0.00257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	879 bp	1.0	78.1
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	CCCGAAAGTTGGATAGGCTG	50.0	55.0	58.1	58.3	891 bp	1.0	77.1
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGAAAGTTGGATAGGCTGG	50.0	55.0	58.1	58.3	890 bp	1.0	77.1
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	ATGCCCGAAAGTTGGATAGG	50.0	50.0	58.1	57.7	894 bp	1.0	77.0
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.2	58.2	921 bp	1.0	76.9