Hotspot

trnT-GGU-psbD

LSC · 74323-75371 · 1049 bp

Back to Alcantarea

Pi 0.00287

MarkerSeek score 60.80

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1049
variable_sites: 3
pi: 0.00287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1205-1209 bp	1.0	79.0
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1208-1212 bp	1.0	78.9
trnT-GGU-psbD_p3	ACGGCCCTTTTAACTCAGTG	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1222-1226 bp	1.0	78.7
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	55.0	45.0	58.1	58.2	1195-1199 bp	1.0	78.0
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.1	58.2	1198-1202 bp	1.0	77.8