Hotspot

trnY-GUA-trnE-UUC

LSC · 73812-73870 · 59 bp

Back to Alcantarea

Pi 0.0169

MarkerSeek score 62.20

Primer available yes

Label trnY-GUA-trnE-UUC

Feature Metadata

label_name: trnY-GUA-trnE-UUC
feature_type: igs
region: LSC
length_bp: 59
variable_sites: 1
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnE-UUC_p1	TGGAGCGGTGAATTATTGGG	GCGTATATGCCCCTATCGTC	50.0	55.0	57.9	57.8	240 bp	1.0	78.3
trnY-GUA-trnE-UUC_p2	TGGAGCGGTGAATTATTGGG	CTCTCTTTCAAGGAGGCAGC	50.0	55.0	57.9	58.3	204 bp	1.0	77.9
trnY-GUA-trnE-UUC_p3	TGGAGCGGTGAATTATTGGG	TCGTCTAGTGGTTCAGGACA	50.0	50.0	57.9	57.7	225 bp	1.0	77.7
trnY-GUA-trnE-UUC_p4	TGGAGCGGTGAATTATTGGG	ATGCCCCTATCGTCTAGTGG	50.0	55.0	57.9	58.4	234 bp	1.0	77.0
trnY-GUA-trnE-UUC_p5	TGGAGCGGTGAATTATTGGG	TGATGATGGTCGGGCGTATA	50.0	50.0	57.9	58.4	253 bp	1.0	76.9