Hotspot

rps19-psbA

LSC · 41821-41965 · 145 bp

Back to Alcantarea

Pi 0.0207

MarkerSeek score 64.40

Primer available yes

Label rps19-psbA

Feature Metadata

label_name: rps19-psbA
feature_type: igs
region: LSC
length_bp: 145
variable_sites: 3
pi: 0.0207
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-psbA_p1	TCTGACTTTCGCGAGACATG	AGCTGTTGTTGAAGCTCCAT	50.0	45.0	58.0	57.7	228 bp	1.0	78.6
rps19-psbA_p2	TCTGACTTTCGCGAGACATG	ATGCTCACAACTTCCCCCTA	50.0	50.0	58.0	58.3	253 bp	1.0	78.2
rps19-psbA_p3	CCTACTCTGACTTTCGCGAG	AGCTGTTGTTGAAGCTCCAT	55.0	45.0	57.8	57.7	233 bp	1.0	77.3
rps19-psbA_p4	CCTACTCTGACTTTCGCGAG	ATGCTCACAACTTCCCCCTA	55.0	50.0	57.8	58.3	258 bp	1.0	76.8
rps19-psbA_p5	TCTGACTTTCGCGAGACATG	TAATGCTCACAACTTCCCCC	50.0	50.0	58.0	57.5	255 bp	1.0	76.7