Hotspot

ccsA-ndhD

IRa · 136062-136630 · 569 bp

Back to Albizia

Pi 0.0686

MarkerSeek score 90.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: IRa
length_bp: 569
variable_sites: 53
pi: 0.0686
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 90.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCAATAGTGGCCTCTATGGG	CAGTTGACAAGGTCGAAGCT	50.0	50.0	56.1	58.1	698-732 bp	1.0	73.5
ccsA-ndhD_p2	TCAATAGTGGCCTCTATGGG	TCAGTTGACAAGGTCGAAGC	50.0	50.0	56.1	58.1	699-733 bp	1.0	73.5
ccsA-ndhD_p3	CTTCAATAGTGGCCTCTATGGG	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	700-734 bp	1.0	72.8
ccsA-ndhD_p4	CTTCAATAGTGGCCTCTATGGG	TCAGTTGACAAGGTCGAAGC	50.0	50.0	58.0	58.1	701-735 bp	1.0	72.7
ccsA-ndhD_p5	TTCAATAGTGGCCTCTATGGG	CAGTTGACAAGGTCGAAGCT	47.6	50.0	56.9	58.1	699-733 bp	1.0	71.7