Hotspot

ndhF-rpl32

SSC · 133297-134280 · 984 bp

Back to Albizia

Pi 0.0601

MarkerSeek score 85.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 984
variable_sites: 84
pi: 0.0601
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CGGGAAAAAGGAGAAGTCCC	TTTGATATAGAAGTGCGTTTTT	55.0	27.3	58.2	52.4	1104-1110 bp	1.0	49.2
ndhF-rpl32_p2	CGGGAAAAAGGAGAAGTCCC	TTTTGATATAGAAGTGCGTTTT	55.0	27.3	58.2	52.4	1105-1111 bp	1.0	49.2
ndhF-rpl32_p3	CGGGAAAAAGGAGAAGTCCC	TTTTTGATATAGAAGTGCGTTT	55.0	27.3	58.2	52.4	1106-1112 bp	1.0	49.2
ndhF-rpl32_p4	CGGGAAAAAGGAGAAGTCCC	TTTTGATATAGAAGTGCGTTTTT	55.0	26.1	58.2	53.3	1105-1111 bp	1.0	49.2
ndhF-rpl32_p5	CGGGAAAAAGGAGAAGTCCC	TTTTTGATATAGAAGTGCGTTTT	55.0	26.1	58.2	53.3	1106-1112 bp	1.0	49.2