Hotspot

rpl16-rps3

LSC · 89754-89906 · 153 bp

Back to Albizia

Pi 0.0389

MarkerSeek score 68.40

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 153
variable_sites: 8
pi: 0.0389
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	CGAGTCGCACACTAAGCAT	ACAGTTCGAACTATTTATGGGGT	52.6	39.1	58.0	57.6	219-235 bp	1.0	51.1
rpl16-rps3_p2	TAAACCAACGCAACGAGTCG	ACAGTTCGAACTATTTATGGGGT	50.0	39.1	59.1	57.6	232-248 bp	1.0	50.1
rpl16-rps3_p3	AACGAGTCGCACACTAAGC	ACAGTTCGAACTATTTATGGGGT	52.6	39.1	58.2	57.6	221-237 bp	1.0	49.9
rpl16-rps3_p4	ATAAACCAACGCAACGAGTC	ACAGTTCGAACTATTTATGGGGT	45.0	39.1	56.8	57.6	233-249 bp	1.0	49.3
rpl16-rps3_p5	CGAGTCGCACACTAAGCAT	TGGGGTATTAGGCATCAAAA	52.6	40.0	58.0	54.3	202-218 bp	1.0	48.9