Hotspot

petA-psbJ

LSC · 68337-69932 · 1596 bp

Back to Albizia

Pi 0.0235

MarkerSeek score 77.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1596
variable_sites: 53
pi: 0.0235
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTCTCTTGTTCTTCGTGGCA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.1	57.6	1702-1723 bp	1.0	82.5
petA-psbJ_p2	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1712-1733 bp	1.0	81.6
petA-psbJ_p3	TGTTCTTCGTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1696-1717 bp	1.0	81.5
petA-psbJ_p4	TTGTTCTTCGTGGCATCTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	1697-1718 bp	1.0	81.5
petA-psbJ_p5	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1717-1738 bp	1.0	81.1