Hotspot

rbcL-accD

LSC · 61889-62795 · 907 bp

Back to Albizia

Pi 0.0235

MarkerSeek score 72.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 907
variable_sites: 32
pi: 0.0235
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	AGTCCTGAATTAGCTGCTGC	TGGGTCTTCACTTACACTGG	50.0	50.0	58.0	56.8	1102-1228 bp	1.0	72.1
rbcL-accD_p2	AGTCCTGAATTAGCTGCTGC	GGGTCTTCACTTACACTGGT	50.0	50.0	58.0	56.8	1101-1227 bp	1.0	72.1
rbcL-accD_p3	GAGGCTAGCAAATGGAGTCC	TGGGTCTTCACTTACACTGG	55.0	50.0	58.0	56.8	1117-1243 bp	1.0	72.0
rbcL-accD_p4	GAGGCTAGCAAATGGAGTCC	GGGTCTTCACTTACACTGGT	55.0	50.0	58.0	56.8	1116-1242 bp	1.0	72.0
rbcL-accD_p5	TTCGTGAGGCTAGCAAATGG	TGGGTCTTCACTTACACTGG	50.0	50.0	58.3	56.8	1122-1248 bp	1.0	70.3