Hotspot

ndhC-trnV-UAC

LSC · 55897-56566 · 670 bp

Back to Albizia

Pi 0.0258
MarkerSeek score 72.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
670
variable_sites
25
pi
0.0258
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGCCCTTTATTAATCGGGG AAAAGATGCACGTTGGGTCT 50.0 45.0 57.3 58.0 888-1241 bp 1.0 78.0
ndhC-trnV-UAC_p2 TGGCCCTTTATTAATCGGGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.3 57.8 817-1170 bp 1.0 76.8
ndhC-trnV-UAC_p3 TGGCCCTTTATTAATCGGGG GATGCACGTTGGGTCTGTAA 50.0 50.0 57.3 58.2 884-1237 bp 1.0 76.6
ndhC-trnV-UAC_p4 TGGCCCTTTATTAATCGGGG ATGCACGTTGGGTCTGTAAA 50.0 45.0 57.3 57.7 883-1236 bp 1.0 76.1
ndhC-trnV-UAC_p5 TGGCCCTTTATTAATCGGGG ACGTTGGGTCTGTAAAGCAA 50.0 45.0 57.3 57.7 879-1232 bp 1.0 75.5