Hotspot

trnC-GCA-petN

LSC · 31240-32367 · 1128 bp

Back to Albizia

Pi 0.0369

MarkerSeek score 68.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 1128
variable_sites: 62
pi: 0.0369
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	1184-1573 bp	1.0	82.1
trnC-GCA-petN_p2	GTATCATTTTGGCGGCATGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.9	58.2	1236-1625 bp	1.0	81.5
trnC-GCA-petN_p3	TCATTCGGAACAGCACATGT	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.8	58.2	1794-2194 bp	1.0	81.3
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	ATAAAGAAAGGGGTTCCGCG	50.0	50.0	58.1	58.3	1623-2012 bp	1.0	80.9
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAG	50.0	55.0	58.1	57.8	1183 bp	0.333333	51.0