Hotspot

psbI-trnS-GCU

LSC · 8677-9031 · 355 bp

Back to Albizia

Pi 0.0446

MarkerSeek score 78.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 355
variable_sites: 22
pi: 0.0446
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CCTATCTAACGATCCAGGACG	TGGACTAAAGCGTCGGATTG	52.4	50.0	57.2	58.0	471-510 bp	1.0	71.1
psbI-trnS-GCU_p2	CCTATCTAACGATCCAGGACG	GTGGACTAAAGCGTCGGATT	52.4	50.0	57.2	58.0	472-511 bp	1.0	71.1
psbI-trnS-GCU_p3	CCTATCTAACGATCCAGGACG	TTTTTCGTACCGAGGGTTCG	52.4	50.0	57.2	58.2	434-473 bp	1.0	69.9
psbI-trnS-GCU_p4	CCTATCTAACGATCCAGGACG	GACTAAAGCGTCGGATTGCT	52.4	50.0	57.2	58.4	469-508 bp	1.0	68.3
psbI-trnS-GCU_p5	CCTATCTAACGATCCAGGACG	CTGAGTGGACTAAAGCGTCG	52.4	55.0	57.2	58.4	476-515 bp	1.0	68.1