Hotspot

trnQ-UUG-psbK

LSC · 7520-8189 · 670 bp

Back to Albizia

Pi 0.0462
MarkerSeek score 80.00
Primer available yes
Label trnQ-UUG-psbK

Feature Metadata

label_name
trnQ-UUG-psbK
feature_type
igs
region
LSC
length_bp
670
variable_sites
43
pi
0.0462
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.95
markerseek_score
80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-psbK_p1 GACCAAAACCCGTTGCCTTA ACAGACCAAACTAAAGATATTAAGCA 50.0 30.8 58.7 56.9 712-737 bp 1.0 48.7
trnQ-UUG-psbK_p2 GACCAAAACCCGTTGCCTTA ACAAATACAGACCAAACTAAAGA 50.0 30.4 58.7 53.9 718-743 bp 1.0 48.7
trnQ-UUG-psbK_p3 GATATGCTCTGGGACGGAAG ACAGACCAAACTAAAGATATTAAGCA 55.0 30.8 57.6 56.9 754-779 bp 1.0 48.5
trnQ-UUG-psbK_p4 GATATGCTCTGGGACGGAAG ACAAATACAGACCAAACTAAAGA 55.0 30.4 57.6 53.9 760-785 bp 1.0 48.4
trnQ-UUG-psbK_p5 AATGGATATGCTCTGGGACG ACAGACCAAACTAAAGATATTAAGCA 50.0 30.8 57.2 56.9 758-783 bp 1.0 48.4