Hotspot

rps16-trnQ-UUG

LSC · 6768-7447 · 680 bp

Back to Albizia

Pi 0.0465

MarkerSeek score 82.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 42
pi: 0.0465
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	694-760 bp	1.0	88.0
rps16-trnQ-UUG_p2	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	696-762 bp	1.0	86.9
rps16-trnQ-UUG_p3	GCACGTTGCTTTTTACCACA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.8	58.1	682-748 bp	1.0	86.5
rps16-trnQ-UUG_p4	CATGTCTTTCAAGTCGCACG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.8	58.1	697-763 bp	1.0	86.3
rps16-trnQ-UUG_p5	GTCTTTCAAGTCGCACGTTG	AAATAAAAATGGGGCGTGGC	50.0	45.0	58.0	57.6	751-817 bp	1.0	85.1