Hotspot

trnK-UUU-rps16

LSC · 4457-5604 · 1148 bp

Back to Albizia

Pi 0.0552
MarkerSeek score 81.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1148
variable_sites
90
pi
0.0552
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCTTTCTGGATCAGTCGTGG AAAAAGGCGCTCAACCTACA 55.0 45.0 58.0 58.0 1380-1623 bp 1.0 87.8
trnK-UUU-rps16_p2 CCTTTCTGGATCAGTCGTGG CCCAATGAGCCGTTTATCGA 55.0 50.0 58.0 58.1 1518-1761 bp 1.0 87.0
trnK-UUU-rps16_p3 CCTTTCTGGATCAGTCGTGG TCCCAATGAGCCGTTTATCG 55.0 50.0 58.0 58.1 1519-1762 bp 1.0 87.0
trnK-UUU-rps16_p4 CCTTTCTGGATCAGTCGTGG TGAGGTTGCGAATACGAGTG 55.0 50.0 58.0 58.0 1724-1968 bp 1.0 86.7
trnK-UUU-rps16_p5 CCTTTCTGGATCAGTCGTGG AAGCTAACGGATCGTGGAAC 55.0 50.0 58.0 58.0 1926-2172 bp 1.0 86.2