Hotspot

ycf41

Genome · 123507-123806 · 300 bp

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Pi 0.024
MarkerSeek score 47.90
Primer available yes
Label ycf41

Feature Metadata

label_name
ycf41
feature_type
gene
region
Genome
length_bp
300
variable_sites
16
pi
0.024
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf41_p1 AGTTGGTCATTGGTGGCATT AGGTCTTTGCTACTGGAAGC 45.0 50.0 58.0 57.5 931 bp 0.2 44.0
ycf41_p2 AGTTGGTCATTGGTGGCATT GGTCTTTGCTACTGGAAGCT 45.0 50.0 58.0 57.5 930 bp 0.2 44.0
ycf41_p3 GCTGCTGCAGGTTTTGAAAA AGGTCTTTGCTACTGGAAGC 45.0 50.0 58.1 57.5 894 bp 0.2 43.7
ycf41_p4 GCTGCTGCAGGTTTTGAAAA GGTCTTTGCTACTGGAAGCT 45.0 50.0 58.1 57.5 893 bp 0.2 43.7
ycf41_p5 TTTACGTGCACCATGGGTAG GGTCTTTGCTACTGGAAGCT 50.0 50.0 57.9 57.5 1164 bp 0.2 43.3