Hotspot

trnT-UGU-trnV-UAC

Genome · 116903-117209 · 307 bp

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Pi 0.0555
MarkerSeek score 76.60
Primer available yes
Label trnT-UGU-trnV-UAC

Feature Metadata

label_name
trnT-UGU-trnV-UAC
feature_type
igs
region
Genome
length_bp
307
variable_sites
35
pi
0.0555
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-trnV-UAC_p1 TTCAAGTCCTTTCACCAGCT GTGACCCTCTGCTTGTAAGG 45.0 55.0 57.0 57.9 376-437 bp 1.0 80.6
trnT-UGU-trnV-UAC_p2 GTTCAAGTCCTTTCACCAGC GTGACCCTCTGCTTGTAAGG 50.0 55.0 56.9 57.9 377-438 bp 1.0 80.2
trnT-UGU-trnV-UAC_p3 TTCAAGTCCTTTCACCAGCT CTGGACTTGAACCAGTGACC 45.0 55.0 57.0 58.1 390-451 bp 1.0 80.1
trnT-UGU-trnV-UAC_p4 GTTCAAGTCCTTTCACCAGC CTGGACTTGAACCAGTGACC 50.0 55.0 56.9 58.1 391-452 bp 1.0 79.8
trnT-UGU-trnV-UAC_p5 TTCAAGTCCTTTCACCAGCT GACTTGAACCAGTGACCCTC 45.0 55.0 57.0 57.8 387-448 bp 1.0 79.7