Hotspot

trnH-GUG-trnT-UGU

Genome · 116749-116829 · 81 bp

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Pi 0.037
MarkerSeek score 70.70
Primer available yes
Label trnH-GUG-trnT-UGU

Feature Metadata

label_name
trnH-GUG-trnT-UGU
feature_type
igs
region
Genome
length_bp
81
variable_sites
7
pi
0.037
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-trnT-UGU_p1 GTTAAGGCAGTGGGTTGTGA AGCTGGTGAAAGGACTTGAA 50.0 45.0 58.0 57.0 210 bp 1.0 74.2
trnH-GUG-trnT-UGU_p2 GTTAAGGCAGTGGGTTGTGA GCTGGTGAAAGGACTTGAAC 50.0 50.0 58.0 56.9 209 bp 1.0 73.9
trnH-GUG-trnT-UGU_p3 TATCAAGGTGGGTGTAGCCA AGCTGGTGAAAGGACTTGAA 50.0 45.0 58.0 57.0 234 bp 1.0 73.8
trnH-GUG-trnT-UGU_p4 TATCAAGGTGGGTGTAGCCA GCTGGTGAAAGGACTTGAAC 50.0 50.0 58.0 56.9 233 bp 1.0 73.5
trnH-GUG-trnT-UGU_p5 TTCAAGTCCCGTCATTCACC AGCTGGTGAAAGGACTTGAA 50.0 45.0 57.8 57.0 174 bp 1.0 73.5