Hotspot

rpl32-trnL-UAG

Genome · 81959-82059 · 101 bp

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Pi 0.0401
MarkerSeek score 73.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
Genome
length_bp
101
variable_sites
8
pi
0.0401
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.97
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAATTGAACAAACTTCAGAAGA ACCTAAATCTAGCGCGTCTG 27.3 50.0 52.4 57.5 170-181 bp 1.0 47.6
rpl32-trnL-UAG_p2 AAAATTGAACAAACTTCAGAAGA ACCTAAATCTAGCGCGTCTG 26.1 50.0 53.3 57.5 171-182 bp 1.0 47.6
rpl32-trnL-UAG_p3 AAATTGAACAAACTTCAGAAGA ACGAACGGAGAGACTTGAAC 27.3 50.0 52.4 57.6 212-223 bp 1.0 46.9
rpl32-trnL-UAG_p4 AAAATTGAACAAACTTCAGAAGA ACGAACGGAGAGACTTGAAC 26.1 50.0 53.3 57.6 213-224 bp 1.0 46.9
rpl32-trnL-UAG_p5 AATTGAACAAACTTCAGAAGAT ACGAACGGAGAGACTTGAAC 27.3 50.0 52.2 57.6 211-222 bp 1.0 46.9