Hotspot

psaI-psbJ

Genome · 77331-77435 · 105 bp

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Pi 0.0419
MarkerSeek score 68.50
Primer available yes
Label psaI-psbJ

Feature Metadata

label_name
psaI-psbJ
feature_type
igs
region
Genome
length_bp
105
variable_sites
8
pi
0.0419
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-psbJ_p1 ACATTGAAAAAGACACTATTGAA TCTGGGTTAGGGTCTTCACT 26.1 50.0 52.8 57.3 155-163 bp 1.0 44.0
psaI-psbJ_p2 TCTACATTGAAAAAGACACTATTGA TCTGGGTTAGGGTCTTCACT 28.0 50.0 54.7 57.3 158-166 bp 1.0 44.0
psaI-psbJ_p3 ACATTGAAAAAGACACTATTGAA TCTGGGTTAGGGTCTTCACTT 26.1 47.6 52.8 58.0 155-163 bp 1.0 44.0
psaI-psbJ_p4 ACATTGAAAAAGACACTATTGAA TTCTGGGTTAGGGTCTTCACT 26.1 47.6 52.8 58.0 156-164 bp 1.0 44.0
psaI-psbJ_p5 TGTTTATCTACATTGAAAAAGACACT TCTGGGTTAGGGTCTTCACT 26.9 50.0 55.6 57.3 164-172 bp 1.0 43.8