Hotspot

clpC-trnK-UUU

Genome · 72631-72772 · 142 bp

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Pi 0.0348
MarkerSeek score 69.60
Primer available yes
Label clpC-trnK-UUU

Feature Metadata

label_name
clpC-trnK-UUU
feature_type
igs
region
Genome
length_bp
142
variable_sites
12
pi
0.0348
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.96
markerseek_score
69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpC-trnK-UUU_p1 GACCTTTACGTCGTGCTCTT TCCGCGTACAGAAGATTGTG 50.0 50.0 57.9 58.0 700-724 bp 1.0 84.1
clpC-trnK-UUU_p2 AATGAATTAGCCTCGCAGGG TCCGCGTACAGAAGATTGTG 50.0 50.0 58.0 58.0 1010-1034 bp 1.0 83.2
clpC-trnK-UUU_p3 TTGGTTTTGGTGGGGAAACA TCCGCGTACAGAAGATTGTG 45.0 50.0 58.0 58.0 988-1012 bp 1.0 83.1
clpC-trnK-UUU_p4 AATGAATTAGCCTCGCAGGG AATGTGTTCGTGCTTGTCCT 50.0 45.0 58.0 58.0 1089-1113 bp 1.0 82.9
clpC-trnK-UUU_p5 AATGAATTAGCCTCGCAGGG CAATGTGTTCGTGCTTGTCC 50.0 50.0 58.0 58.0 1090-1114 bp 1.0 82.8