Hotspot

trnG-GCC-psbI

Genome · 36020-36142 · 123 bp

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Pi 0.0569
MarkerSeek score 75.20
Primer available yes
Label trnG-GCC-psbI

Feature Metadata

label_name
trnG-GCC-psbI
feature_type
igs
region
Genome
length_bp
123
variable_sites
15
pi
0.0569
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbI_p1 GTTGCGCTCTACCACTGAG CCATCAAGAAACCCAAACCG 57.9 50.0 58.3 57.3 188-198 bp 1.0 70.3
trnG-GCC-psbI_p2 CAAGGTTGCGCTCTACCAC CCATCAAGAAACCCAAACCG 57.9 50.0 58.8 57.3 192-202 bp 1.0 65.6
trnG-GCC-psbI_p3 AAGGTTGCGCTCTACCACT CCATCAAGAAACCCAAACCG 52.6 50.0 58.9 57.3 191-201 bp 1.0 64.8
trnG-GCC-psbI_p4 CTCTACCACTGAGCTACACTC CCATCAAGAAACCCAAACCG 52.4 50.0 57.0 57.3 182-192 bp 1.0 64.2
trnG-GCC-psbI_p5 GCGACATCAACCTTGGCAA CCATCAAGAAACCCAAACCG 52.6 50.0 59.0 57.3 208-218 bp 1.0 63.5