Hotspot

secA-ycf54

Genome · 34165-34411 · 247 bp

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Pi 0.0498
MarkerSeek score 78.40
Primer available yes
Label secA-ycf54

Feature Metadata

label_name
secA-ycf54
feature_type
igs
region
Genome
length_bp
247
variable_sites
27
pi
0.0498
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
secA-ycf54_p1 TAACGCGGGTGAAGTTGAAA TTGCGTGAACGAGTACAACA 45.0 45.0 58.0 58.1 861-871 bp 1.0 84.7
secA-ycf54_p2 TAACGCGGGTGAAGTTGAAA TGCGTGAACGAGTACAACAT 45.0 45.0 58.0 57.9 860-870 bp 1.0 84.2
secA-ycf54_p3 ACCCATCCATTCTGCATCAC TTGCGTGAACGAGTACAACA 50.0 45.0 57.9 58.1 942-952 bp 1.0 84.1
secA-ycf54_p4 ACCCATCCATTCTGCATCAC TGCGTGAACGAGTACAACAT 50.0 45.0 57.9 57.9 941-951 bp 1.0 83.6
secA-ycf54_p5 TAACGCGGGTGAAGTTGAAA TGTTCAAGCGGGTAGACAAG 45.0 50.0 58.0 57.8 1278-1288 bp 1.0 83.2