Hotspot

trnS-UGA-psaD

Genome · 24802-25005 · 204 bp

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Pi 0.049
MarkerSeek score 70.20
Primer available yes
Label trnS-UGA-psaD

Feature Metadata

label_name
trnS-UGA-psaD
feature_type
igs
region
Genome
length_bp
204
variable_sites
20
pi
0.049
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psaD_p1 ACGCGTAAACGTTCATCTGA CTCATAACCCGAAAGTCGCA 45.0 50.0 57.9 58.0 717-758 bp 1.0 87.5
trnS-UGA-psaD_p2 ACGCGTAAACGTTCATCTGA TCATAACCCGAAAGTCGCAG 45.0 50.0 57.9 58.0 716-757 bp 1.0 87.5
trnS-UGA-psaD_p3 GGGTTGCTGGGAACACTAAA CTCATAACCCGAAAGTCGCA 50.0 50.0 58.0 58.0 769-810 bp 0.4 61.8
trnS-UGA-psaD_p4 GGGTTGCTGGGAACACTAAA TCATAACCCGAAAGTCGCAG 50.0 50.0 58.0 58.0 768-809 bp 0.4 61.8
trnS-UGA-psaD_p5 GGGTTGCTGGGAACACTAAA CGCAGTTTGGTAGCGTATCT 50.0 50.0 58.0 58.1 913-954 bp 0.4 60.6