Hotspot

petD-atpA

Genome · 8643-8731 · 89 bp

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Pi 0.0693
MarkerSeek score 77.40
Primer available yes
Label petD-atpA

Feature Metadata

label_name
petD-atpA
feature_type
igs
region
Genome
length_bp
89
variable_sites
11
pi
0.0693
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.84
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petD-atpA_p1 AGGAATTGGAGCTTGTTTACCA AAAAGTGCAATAGATGACGT 40.9 35.0 57.9 53.2 172-186 bp 1.0 49.0
petD-atpA_p2 AGGAATTGGAGCTTGTTTACCA AAAAAGTGCAATAGATGACGT 40.9 33.3 57.9 54.1 173-187 bp 1.0 49.0
petD-atpA_p3 TGGTTAGGAATTGGAGCTTGT AAAAGTGCAATAGATGACGT 42.9 35.0 57.2 53.2 177-191 bp 1.0 48.9
petD-atpA_p4 TGGTTAGGAATTGGAGCTTGT AAAAAGTGCAATAGATGACGT 42.9 33.3 57.2 54.1 178-192 bp 1.0 48.9
petD-atpA_p5 TGGTTAGGAATTGGAGCTTGTT AAAAGTGCAATAGATGACGT 40.9 35.0 57.9 53.2 177-191 bp 1.0 48.9