Hotspot

ycf1

SSC · 126287-131758 · 5472 bp

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Pi 0.00435

MarkerSeek score 32.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5472
variable_sites: 63
pi: 0.00435
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGAAGTAATTTGCTTGGTATAACCC	AAATACTAAGACCGGCACCA	36.0	45.0	57.4	56.5	258 bp	1.0	41.6
ycf1_p2	AGAAGTAATTTGCTTGGTATAACCC	AATACTAAGACCGGCACCAA	36.0	45.0	57.4	56.5	257 bp	1.0	41.6
ycf1_p3	AGAAGTAATTTGCTTGGTATAACCC	ACTAAGACCGGCACCAATAA	36.0	45.0	57.4	56.5	254 bp	1.0	41.6
ycf1_p4	AGTAATTTGCTTGGTATAACCC	AATACTAAGACCGGCACCAA	36.4	45.0	54.1	56.5	254 bp	1.0	41.6
ycf1_p5	AGTAATTTGCTTGGTATAACCC	ACTAAGACCGGCACCAATAA	36.4	45.0	54.1	56.5	251 bp	1.0	41.6