Hotspot

ndhA

SSC · 122160-124366 · 2207 bp

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Pi 0.00281

MarkerSeek score 53.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2207
variable_sites: 16
pi: 0.00281
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	58.1	57.9	2321-2326 bp	1.0	71.2
ndhA_p2	ACCCAGTTACCATAGGGAACA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	58.1	57.8	2319-2324 bp	1.0	70.6
ndhA_p3	TGAACCCAGTTACCATAGGGA	ATGGGGGAAGTTGATCGTTG	47.6	50.0	57.8	57.9	2324-2329 bp	1.0	70.1
ndhA_p4	TGAACCCAGTTACCATAGGGA	GGGGGAAGTTGATCGTTGAA	47.6	50.0	57.8	57.8	2322-2327 bp	1.0	69.6
ndhA_p5	CCCAGTTACCATAGGGAACA	ATGGGGGAAGTTGATCGTTG	50.0	50.0	56.2	57.9	2320-2325 bp	1.0	65.5