Hotspot

ndhD-psaC

SSC · 119572-119671 · 100 bp

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Pi 0.048

MarkerSeek score 57.10

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 100
variable_sites: 9
pi: 0.048
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	CGTGGTAAAGACAAGATAGGGT	AAGATGTGAATCCGCCTGTC	45.5	50.0	57.5	58.0	221-222 bp	1.0	63.3
ndhD-psaC_p2	AGACAAGATAGGGTTGAACCAG	AAGATGTGAATCCGCCTGTC	45.5	50.0	57.4	58.0	213-214 bp	1.0	62.7
ndhD-psaC_p3	AGACAAGATAGGGTTGAACCA	AAGATGTGAATCCGCCTGTC	42.9	50.0	56.3	58.0	213-214 bp	1.0	61.3
ndhD-psaC_p4	TCGTGGTAAAGACAAGATAGGG	AAGATGTGAATCCGCCTGTC	45.5	50.0	57.3	58.0	222-223 bp	1.0	61.2
ndhD-psaC_p5	CGTGGTAAAGACAAGATAGGGT	CAGAGGACTGTGTTGGTTGT	45.5	50.0	57.5	57.7	243-244 bp	1.0	60.6