Hotspot

rpl32-trnL-UAG

SSC · 115735-116649 · 915 bp

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Pi 0.0039

MarkerSeek score 50.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 915
variable_sites: 9
pi: 0.0039
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GCGCTAAAAGCCTTTTCGTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.0	58.0	1022-1025 bp	1.0	80.6
rpl32-trnL-UAG_p2	GCGCTAAAAGCCTTTTCGTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.0	58.0	1023-1026 bp	1.0	80.6
rpl32-trnL-UAG_p3	GAAGAAGGGGTATTGGGCAG	AGCGTGTCTACCGATTTCAC	55.0	50.0	57.9	58.0	1041-1044 bp	1.0	80.5
rpl32-trnL-UAG_p4	GAAGAAGGGGTATTGGGCAG	CAGCGTGTCTACCGATTTCA	55.0	50.0	57.9	58.0	1042-1045 bp	1.0	80.5
rpl32-trnL-UAG_p5	GCGCTAAAAGCCTTTTCGTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	1032-1035 bp	1.0	79.6