Hotspot

ndhF-rpl32

SSC · 114373-115572 · 1200 bp

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Pi 0.00717

MarkerSeek score 52.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1200
variable_sites: 22
pi: 0.00717
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CCAAAGGTATGATCCACGCA	AACGAAAAGGCTTTTAGCGC	50.0	45.0	58.0	58.0	1341-1344 bp	1.0	80.6
ndhF-rpl32_p2	ACCAAAGGTATGATCCACGC	AACGAAAAGGCTTTTAGCGC	50.0	45.0	58.0	58.0	1342-1345 bp	1.0	80.6
ndhF-rpl32_p3	CCAAAGGTATGATCCACGCA	CTGCCCAATACCCCTTCTTC	50.0	55.0	58.0	57.9	1322-1325 bp	1.0	80.6
ndhF-rpl32_p4	ACCAAAGGTATGATCCACGC	CTGCCCAATACCCCTTCTTC	50.0	55.0	58.0	57.9	1323-1326 bp	1.0	80.6
ndhF-rpl32_p5	TCGGAAAAAGAAGAAGCCCC	AACGAAAAGGCTTTTAGCGC	50.0	45.0	58.1	58.0	1392-1395 bp	1.0	80.1