Hotspot

rrn16-trnI-GAU

IRb · 103949-104243 · 295 bp

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Pi 0.0174

MarkerSeek score 38.90

Primer available yes

Label rrn16-trnI-GAU

Feature Metadata

label_name: rrn16-trnI-GAU
feature_type: igs
region: IRb
length_bp: 295
variable_sites: 9
pi: 0.0174
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnI-GAU_p1	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	55.0	50.0	57.7	58.2	388 bp	1.0	78.0
rrn16-trnI-GAU_p2	AGTGACTGGAGTGAAGTCGT	GGCACAACGACGCAATTATC	50.0	50.0	58.3	58.2	410 bp	1.0	77.8
rrn16-trnI-GAU_p3	AAGTCGTAACAAGGTAGCCG	GGCACAACGACGCAATTATC	50.0	50.0	57.6	58.2	397 bp	1.0	77.4
rrn16-trnI-GAU_p4	AACAAGGTAGCCGTACTGGA	GGCACAACGACGCAATTATC	50.0	50.0	58.4	58.2	390 bp	1.0	77.4
rrn16-trnI-GAU_p5	ACAAGGTAGCCGTACTGGAA	GGCACAACGACGCAATTATC	50.0	50.0	58.4	58.2	389 bp	1.0	77.4