Hotspot

rpl20-rps12

LSC · 70882-71670 · 789 bp

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Pi 0.00211

MarkerSeek score 52.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 789
variable_sites: 5
pi: 0.00211
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTTGAATGAGCCCCTCGAAA	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	899-909 bp	1.0	74.6
rpl20-rps12_p2	TTGAATGAGCCCCTCGAAAG	CAGCGTCGAGGAACATGTAC	50.0	55.0	57.9	58.7	898-908 bp	1.0	74.6
rpl20-rps12_p3	CTTGAATGAGCCCCTCGAAA	AGCGTCGAGGAACATGTACT	50.0	50.0	57.9	58.8	898-908 bp	1.0	73.8
rpl20-rps12_p4	TTGAATGAGCCCCTCGAAAG	AGCGTCGAGGAACATGTACT	50.0	50.0	57.9	58.8	897-907 bp	1.0	73.8
rpl20-rps12_p5	TTGAATGAGCCCCTCGAAAG	TCAGCGTCGAGGAACATGTA	50.0	50.0	57.9	58.8	899-909 bp	1.0	73.8