Hotspot

trnF-GAA-ndhJ

LSC · 49698-50384 · 687 bp

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Pi 0.0065

MarkerSeek score 48.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 687
variable_sites: 11
pi: 0.0065
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	828-829 bp	1.0	80.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	792-793 bp	1.0	80.5
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	863-864 bp	1.0	79.9
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	816-817 bp	1.0	79.8
trnF-GAA-ndhJ_p5	GTCAATGAGGCTTCGGGAAT	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	853 bp	0.833333	73.1