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trnC-GCA-petN

LSC · 28924-29506 · 583 bp

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Pi 0.0064

MarkerSeek score 44.20

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 10
pi: 0.0064
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTTGTATCGTTTTGGCGGC	AGCCCAAGCCAGACTTACTA	45.0	50.0	57.9	58.0	705-706 bp	1.0	80.3
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCCAGACTTACTA	50.0	50.0	58.1	58.0	649-650 bp	1.0	80.2
trnC-GCA-petN_p3	GGTGTCGCCTGATCAAGAAA	AGCCCAAGCCAGACTTACTA	50.0	50.0	58.2	58.0	630-631 bp	1.0	79.4
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGCCCAAGCCAGACTTACTA	50.0	50.0	58.4	58.0	667-668 bp	1.0	78.0
trnC-GCA-petN_p5	TTTTGTATCGTTTTGGCGGC	AGAGTCCACTTCTTCCCCAT	45.0	50.0	57.9	57.6	766-767 bp	1.0	77.8