Hotspot

trnS-GCU-trnG-UCC

LSC · 9006-9744 · 739 bp

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Pi 0.00595

MarkerSeek score 48.00

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 739
variable_sites: 12
pi: 0.00595
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TGAATCAAACCAAAAGACCCCT	50.0	40.9	58.0	58.1	852-856 bp	1.0	64.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	TGAATCAAACCAAAAGACCCCT	50.0	40.9	58.0	58.1	851-855 bp	1.0	64.7
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACCAAAAGACCCCT	50.0	40.9	58.2	58.1	906-910 bp	1.0	63.5
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	TGAATCAAACCAAAAGACCCC	50.0	42.9	58.0	56.6	852-856 bp	1.0	62.2
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	TGAATCAAACCAAAAGACCCC	50.0	42.9	58.0	56.6	851-855 bp	1.0	62.2