Hotspot

psaB

LSC · 39309-41513 · 2205 bp

Pi 0.00068

MarkerSeek score 48.40

Primer available yes

Label psaB

Feature Metadata

label_name: psaB
feature_type: gene
region: LSC
length_bp: 2205
variable_sites: 3
pi: 0.00068
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaB_p1	ATAAGAAGGTGGACCCCCTT	ACAACATGGGCGTTCTTCTT	50.0	45.0	57.6	58.0	2352-2355 bp	1.0	77.0
psaB_p2	CCCTTCTACGTCGAACCAAA	ACAACATGGGCGTTCTTCTT	50.0	45.0	57.6	58.0	2337-2340 bp	1.0	76.5
psaB_p3	ATAAGAAGGTGGACCCCCTT	TACAAGGACGTGCTGTAGGA	50.0	50.0	57.6	58.1	2402-2405 bp	1.0	76.5
psaB_p4	ATAAGAAGGTGGACCCCCTT	GGGCGTTCTTCTTAGCAAGA	50.0	50.0	57.6	57.9	2345-2348 bp	1.0	76.4
psaB_p5	CCCTTCTACGTCGAACCAAA	TACAAGGACGTGCTGTAGGA	50.0	50.0	57.6	58.1	2387-2390 bp	1.0	75.9