Hotspot

rpl32-trnL-UAG

SSC · 116468-117449 · 982 bp

Back to Akebia

Pi 0.00866
MarkerSeek score 61.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
982
variable_sites
17
pi
0.00866
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TGGACAGCGGTAAAAGCTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 1109-1114 bp 1.0 81.3
rpl32-trnL-UAG_p2 TTGGACAGCGGTAAAAGCTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.0 58.0 1110-1115 bp 1.0 81.3
rpl32-trnL-UAG_p3 TGGACAGCGGTAAAAGCTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 1110-1115 bp 1.0 81.3
rpl32-trnL-UAG_p4 TTGGACAGCGGTAAAAGCTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.0 58.0 1111-1116 bp 1.0 81.3
rpl32-trnL-UAG_p5 TGGACAGCGGTAAAAGCTTT TTTAGAAGATGCCGCCACTC 45.0 50.0 58.0 58.0 1169-1174 bp 1.0 81.1